PRACTICAL 3: MICROORGANISMS FROM THE ENVIRONMENT AND BODY SURFACES

PRACTICAL 3: MICROORGANISMS FROM THE ENVIRONMENT AND BODY SURFACES Floor plan of laboratory and position of Nutrient Agar plates Time exposed to air 24 hours

Laboratory floor plan and nutrient agar plate placement The exposure time is 24 hours. Then it was incubated for 48 hours at 37 degrees Celsius.

PRACTICAL 3: MICROORGANISMS FROM THE ENVIRONMENT AND BODY SURFACES Part 1 Results: Air Plates

After incubation of the agar plates, observe the colonies.  Draw and describe the appearance (shapes, diameter sizes in mm, colours) of the colonies here: Microbial colonies on Air Plates (1 mark)

Location in the lab	Shape	Sizes	Colour
1.     By lecturer computer	Circular, opaque
2.     Top of the bin
3.     Under the table just above the floor
4.     Corner of the class room on the cupboard 2m high
5.     Top of an incubator, around 1,5m high, close to a frequently used door entry

Which plates attracted more microorganisms than others and where were they positioned in the laboratory (2 marks)? ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. Why do you think these plates attracted more microorganisms than others (2 marks)? ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. …………………………………………………………………………………………..

Part 2 Results: Environmental Swabs

After incubation of the agar plates, observe the colonies.  Draw and describe the appearance (shapes, diameter sizes in mm, colours) of individual colonies on the two types of agar plates: Microorganisms from the environment

Type of surface swabbed:     Was the 10×10 cm template used? Y/N	Type of surface swabbed:     Was the 10×10 cm template used? Y/N
Colony shapes, sizes (in mm) and colours   NA plate	Colony shapes, sizes (in mm) and colours   MEA plate

Class results (1 marks)

Location in the lab	shape	sizes	colour

Do any particular surfaces appear to be more contaminated with microorganisms than others (1 mark)? ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. Why should this be so (2 mark)? ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. Do the colonies on Nutrient Agar and Malt Extract Agar look different and in what way (2 mark)? ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. If so, what does this say about the type of microorganisms present on the surface you have swabbed (2 mark)? ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. …………………………………………………………………………………………..

PRACTICAL 3: MICROORGANISMS FROM THE ENVIRONMENT AND BODY SURFACES Part 3 Results: Body Swabs

After incubation of the agar plates, observe the colonies.  Draw and describe the appearance (shapes, diameter sizes in mm, colours) of the colonies here:  Microorganisms from body surfaces (1 mark)

Part of body swabbed: ………………………………………	Part of body swabbed: ………………………………………
Colony shapes, sizes (in mm) and colours   NA plate	Colony shapes, sizes (in mm) and colours   BPA plate

Photo for the two agar plates Compare and discuss your results with your classmates. Do any particular body parts support more microbial growth than others (2 mark)? ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. Why should this be so(1 mark)? ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. …………………………………………………………………………………………..     What does this tell you about the importance of personal hygiene (2 mark)? ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. …………………………………………………………………………………………..   Do the colonies on Nutrient Agar and Baird-Parker Agar look different (colour, shape)?  Why should this be so?  (1 marks)   ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. ………………………………………………………………………………………….. Total marks 20

ADDITIONAL INSTRUCTIONS FOR THE CLASS –

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• Discussion Questions (DQ)

Initial responses to the DQ should address all components of the questions asked, including a minimum of one scholarly source, and be at least 250 words. Successful responses are substantive (i.e., add something new to the discussion, engage others in the discussion, well-developed idea) and include at least one scholarly source. One or two-sentence responses, simple statements of agreement or “good post,” and responses that are off-topic will not count as substantive. Substantive responses should be at least 150 words. I encourage you to incorporate the readings from the week (as applicable) into your responses.

• Weekly Participation

Your initial responses to the mandatory DQ do not count toward participation and are graded separately. In addition to the DQ responses, you must post at least one reply to peers (or me) on three separate days, for a total of three replies. Participation posts do not require a scholarly source/citation (unless you cite someone else’s work). Part of your weekly participation includes viewing the weekly announcement and attesting to watching it in the comments. These announcements are made to ensure you understand everything that is due during the week.

• APA Format and Writing Quality

Familiarize yourself with the APA format and practice using it correctly. It is used for most writing assignments for your degree. Visit the Writing Center in the Student Success Center, under the Resources tab in Loud-cloud for APA paper templates, citation examples, tips, etc. Points will be deducted for poor use of APA format or absence of APA format (if required). Cite all sources of information! When in doubt, cite the source. Paraphrasing also requires a citation. I highly recommend using the APA Publication Manual, 6th edition.

• Use of Direct Quotes

I discourage over-utilization of direct quotes in DQs and assignments at the Master’s level and deduct points accordingly. As Masters’ level students, it is important that you be able to critically analyze and interpret information from journal articles and other resources. Simply restating someone else’s words does not demonstrate an understanding of the content or critical analysis of the content. It is best to paraphrase content and cite your source. PRACTICAL 3: MICROORGANISMS FROM THE ENVIRONMENT AND BODY SURFACES

• LopesWrite Policy

For assignments that need to be submitted to Lopes Write, please be sure you have received your report and Similarity Index (SI) percentage BEFORE you do a “final submit” to me. Once you have received your report, please review it. This report will show you grammatical, punctuation, and spelling errors that can easily be fixed. Take the extra few minutes to review instead of getting counted off for these mistakes. Review your similarities. Did you forget to cite something? Did you not paraphrase well enough? Is your paper made up of someone else’s thoughts more than your own? Visit the Writing Center in the Student Success Center, under the Resources tab in Loud-cloud for tips on improving your paper and SI score.

• Late Policy

The university’s policy on late assignments is a 10% penalty PER DAY LATE. This also applies to late DQ replies. Please communicate with me if you anticipate having to submit an assignment late. I am happy to be flexible, with advance notice. We may be able to work out an extension based on extenuating circumstances. If you do not communicate with me before submitting an assignment late, the GCU late policy will be in effect. I do not accept assignments that are two or more weeks late unless we have worked out an extension. As per policy, no assignments are accepted after the last day of class. Any assignment submitted after midnight on the last day of class will not be accepted for grading. PRACTICAL 3: MICROORGANISMS FROM THE ENVIRONMENT AND BODY SURFACES

• Communication

Communication is so very important. There are multiple ways to communicate with me: Questions to Instructor Forum: This is a great place to ask course content or assignment questions. If you have a question, there is a good chance one of your peers does as well. This is a public forum for the class. Individual Forum: This is a private forum to ask me questions or send me messages. This will be checked at least once every 24 hours.

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